Boiling Process

1. To a clean beaker, add 1.5g of sodium chloride, 1.464 g of Span 60, and 1.288 g of TPGS in 50mL of PBS, and a stirrer bar.
• This will result in 50 mL of solution, can multiply each ingredient by 2 to get 100 mL, and so on.
• The smaller the pieces of TPGS, the less time for it to melt

2. Cover this beaker with aluminum foil and put a piece of “autoclave” tape on the foil.

3. Heat/stir this beaker (not too hot) until you see it begin boiling. 

4. Let the solution boil until Span and TPGS completely melt/dissolve, approximately 1-2 hours.

5. Autoclave for approximately 35 min. This reduces Span60 particle size.  ***